–  Hichner B1/Lasota strain cultured on Embryonated SPF eggs

– Freeze – dried (Lyophilized) vaccine with a minimum titer of 106.5  EID50 (Embryo infective Dose) 50


Vaccine should be stored at a temperature of -20 ºC


– Reconstitute according to the number of chicken to be vaccinated and dilute the required amount of vaccine.

– The water must be free from antiseptics (Well or spring water).

– The chicken will not be given drinking water the evening before vaccination day.


Lasota and Hichner B1

OCULAR ROUTE – Use an eye dropper. To calculate the volume of water which should be added to dilute the number of doses of the vaccine per vial follow the instructions below:

Measure 1 ml of water to the dropper

Count the number of drops in this 1 ml of water

Calculate the volume of diluent required to dilute the number of doses of the vaccine per vial with the eye – dropper in use:

Volume of diluent (ml) = No. of doses of vaccine per vial
No. of drops formed per ml
Example: How much diluent should be added to a vial containing 250 doses of ND vaccine given that 1 ml of water in the eye – dropper yielded 50 drops?
Volume of diluent (ml) = 250 doses per vial
50 drops per ml
= 5 ml per vial

ORAL DRENCH – Dissolve the 200 doses in 200 ml, the 100 doses in 100 ml and the 50 doses in 50 ml. administer by oral drench 1 ml of dissolved vaccine squirting into the beak of each bird using a clean plastic syringe.

DRINKING WATER – the quantity of water generally required per bird for the drinking water vaccination is as follows:

 FOR 10 – 14 DAY – OLD BIRDS………………………………….10-15 ML
FOR 3 – 8 WEEKS – OLD BIRDS……………………………….20-30 ML
FOR OTHER BIRDS………………………………………………….40 ML

To calculate the volume of water required to dilute the vaccine, multiply the number of doses of the vaccine per vial by the amount of ml required per bird according to the above table.

Example: to dilute 500 doses of vaccine for 8 week – old birds multiply 500 by 30 that means you need 15 liters of water to dilute the 500 doses of vaccine per vial.


– First vaccination: Day old and Above (preferably up to 10 days)
– Second vaccination: 3 – 4 weeks.
– Third vaccination: 3 – 4 months.
– Repeat every 3 – 4 months.
For example, in flcks of chicks having high level of maternal antibody, it has been found that approx.60% of the chicks will actively respond
to vaccination at 18 – 21 days of age, while at 14 days of age the percentage
of chicks that will respond may be under 40%.
In the case of broilers in low risk areas, revaccination at 14 – 21 days of
age will prove adequate protection.
In high risk areas application of vaccine at this age only may be inadequate.
Therefore, it must be necessary to revaccinate at 42 days of age using the Lasota vaccine by the drinking water. In the case of layers, further boosting of immunity is necessary after 10 weeks of age. This is to protect the birds from the disease during the remainder of the growing period, and to provide at point of lay an immune level that will effctively protect the pullets with the minimum of revaccination during the laying period.
If the time interval between the primary and secondary vaccination is < 21 days, the antibody produced by the fist dose of vaccine is more likely to interfere with the multiplication of the second dose of virus.
An interval during which no further vaccine is given should be allowed
until the fial dose of vaccine is administered about two weeks before the
birds come in to egg production.
Day of Vaccination                 Type of vaccine        Route of administration
Day 7                                            HB1                              Intraocular
Day 21                                          Lasota                          Intraocular
Day 42                                          Lasota                          by the drinking water
Between 10 and 12

weeks of age                               Inactivated                  Intramuscular 0.5ml/bird
At point of egg lay                      Inactivated                  Intramuscular0.5ml/bird

Vaccinate only apparently healthy chickens